Determination of Parishin, Parishins B and C in Gastrodiae Rhizoma by HPLC

YOE-RAY KU, YI-CHU LIU, JING-PING HAU, KUO-CHING WEN, JER-HUEI LIN AND *WENG-FONG HUANG

National Laboratories of Foods and drugs, Department of Health, Executive Yuan 161-2, Kuen Yang Street, Nankang, Taipei, Taiwan, R.O.C.
*Graduate Institute of Health & Welfare Policy, National Yang-Ming University, Shih-Pai, Taipei 11221, Taiwan, R.O.C.

ABSTRACT

   A rapid method by high performance liquid chromatography was developed for the simultaneous determination of three marker substances, parishin, parishins B and C, in Gastrodiae Rhizoma. This determination was carried out by using a gradient elution with methanol and 0.1% phosphoric acid on a Cosmosil 5C18-AR column. The linear calibration range of parishin, parishins B and C were 80~1200ug/ml (r=0.9998), 120~760ug/ml (r=0.9831) and 40~320 ug/ml(r=0.9995), respectively. The recoveries of the markers were parishin: 102.6±3.3%, parishin B: 102.8±2.7% and parishin C: 102.6± 2.9%. The relative standard deviations of the three marker substances for intraday and interday analyses were 0.73~2.11% and 0.67~3.56%, respectively. The contents of the markers mentioned above in 23 crude drugs of Castrodiae Rhizoma have been determined and the results were obtained as follows : parishin 0.37~1.50%, parishin B 0.12~0.65% and parishin C 0.08~0.27%. Therefore, HPLC is a useful technique to determine these selected markers.

Key words :Gastrodiae Rhizoma, Gastrodia elata Blume, parishin, parishin B, parishin C,HPLC