Determination of Seven Pueraria Constituents by High Performance Liquid Chromatography and Capillary Electrophoresis
CHING-CHE LIN1, MING-HSING HUANG2 AND SHUENN-JYI SHEU1*
1. Department of Chemistry, National Taiwan Normal University, 88, Sec. 4, Tingchow Rd., Taipei City 116, Taiwan, R.O.C
2. Department of Applied Life Science and Health, Chia Nan University of Pharmacy and Science,
60, Erh-Jen Rd., Sec.1, Jan-Te Township, Tainan County 717, Taiwan, R.O.C
(Received: March 2, 2005; Accepted: August 9, 2005)
ABSTRACT
Puerariae Radix is a commonly used C hinese herbal medicine derived from the dried root of the legume plant, which contains a series of isoflavones as its chief pharmacologically active constituents. U sing 7 pueraria constituents as marker substances, a high performance liquid chromatography (HPLC) and a capillary electrophoresis (CE) method were developed to assay the quality of Puerariae Radix within 65 and 50 min, respectively. Extracted samples were analyzed with a C osmosil 5C18-MS column and eluted with a mixture of potassium dihydrogenphosphate (30 mM, adjusted to pH 3.0 with phosphoric acid) and aqueous acetonitrile solution, or an uncoated capillary (75 μm I.D.) and carried with an aqueous solution containing 10 mM sodium dihydrogenphosphate and 40 mM sodium dodecyl sulphate. The relative standard deviation of the marker substances, on the basis of peak-area ratios for 6 replicate injections, was 0.50~0.97% (intraday) and 0.48~0.97% (interday) for HPLC, and 0.88~1.25% (intraday) and 0.85~1.38% (interday) for C E. Both methods yielded accurate results differing by only 5% when applied to the analysis of practical samples of Puerariae Radix.
Key words: pueraria constituents, high performance liquid chromatography (HPLC), capillary electrophoresis (CE)