Determination of Adenosine, Cordycepin and Ergosterol Contents in Cultivated Antrodia camphorata by HPLC Method
CHIEN-YU CHANG1,2, MING-YONG LUE3 AND TZU-MING PAN1*
1. Institute of Microbiology and Biochemistry, National Taiwan University, 1, Sec. 4, Roosevelt Rd., Taipei City 115, Taiwan, R.O.C.
2. Taiwan Sugar Research Institute, Tainan, Taiwan, R.O.C.
3. Department of Cosmetic Science, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan, R.O.C.
(Received: March 3, 2005; Accepted: May 26, 2005)
ABSTRACT
The concentrations of adenosine and cordycepin, 3'-deoxyadenosine in the hot water extract and ergosterol in the ethanol extract of a cultivated Antrodia camphorata were measured by high performance liquid chromatography (HPLC) method. The procedure were carried out on a reversed-phase C18 column by linear gradient elution, using four eluents as mobile phase and by isocratic elution, using 100% methanol as mobile phase, respectively. The analysis was completed within 20 min. The application of this method for quantitation of adenosine and cordycepin in 5-L and 100-L fermentation from two A. camphorata strains BCRC 35396 and BCRC 35398 were described. C omparison of adenosine, cordycepin and ergosterol contents were made with different reatment of 700-L fermentation broth.
Key words: adenosine, cordycepin, ergosterol, Antrodia camphorata, high performance liquid chromatography (HPLC) method