A Removable Derivatization HPLC for Analysis of Methanol in Chinese Liquor Medicine
CHIA-CHI KUO 1, YEN-HSIA WEN 1, CHIEH-MING HUANG 2, HSIN-LUNG WU 1 AND SHIHN-SHENG Wu 1*
1. Graduate Institute of Pharmaceutical Sciences and School of Pharmacy, Kaohsiung Medical University, 100 Shin Chen 1St Rd., Kaohsiung 807, Taiwan, R.O.C
2. Kaohsiung Municipal Chinese Medical Hospital, 6 Fu Cheng Street, Ling Ya District, Kaohsiung Taiwan, R.O.C
(Received: June 15, 2001; Accepted: November 2, 2001)
ABSTRACT
A high performance liquid chromatography (HPLC) method for the determination of trace methanol in high water content sample has been established. Using 4-[N-methyl, N-(1-naphthylmethyl)]-amino-4-oxobutanoic acid (NAOB), 4-dimethylaminopyridine (DMAP) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) under a mild condition, the derivatization of methanol in aqueous solution was smoothly carried out before HPLC analysis. After derivatization, acidic or basic interfering substances and excess reagents can be easily removed from organic layer by simple acid-base shake-out. The condition for optimal derivatization of methanol in aqueous solution was established and applicable to the analysis of commercial Chinese liquor medicine (ethanol content 8~55%, v/v), the range of trace methanol in the above samples was 2.20 x 10-4~ 3.28 x 10-3% (v/v). The result showed that methanol contents in all samples were below regulation standard (< 0.1%, v/v).
Keywords: removable derivatization, methanol, HPLC, Chinese liquor medicine