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4. Polysaccharide-Rich Red Algae (Gelidium amansii) Hot-Water Extracts Ameliorate the Altered Plasma Cholesterol and Hepatic Lipid Homeostasis in High-Fat Diet-Fed Rats 【發布日期:2021-04-15】 發布單位:

Polysaccharide-Rich Red Algae (Gelidium amansii) Hot-Water Extracts Ameliorate the Altered Plasma Cholesterol and Hepatic Lipid Homeostasis in High-Fat Diet-Fed Rats

Shing-Hwa Liu a,b,c, Hsin-I Hung d, Tsung-Han Yang d, Chorng-Liang Pan d, Meng-Tsan Chiang d,*

a Graduate Institute of Toxicology, College of Medicine, National Taiwan University, Taipei 10051, Taiwan

b Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 40402, Taiwan

c Department of Pediatrics, College of Medicine, National Taiwan University Hospital, Taipei 10051, Taiwan

d Department of Food Science, National Taiwan Ocean University, Keelung 20224, Taiwan

We have demonstrated that red algae Gelidium amansii (GA) hot-water extract (GHE) is a polysaccharide-rich fraction, containing 68.54% water-soluble indigestible carbohydrate polymers; the molecular weight of major polysaccharide is 892. Here, we investigated the mechanisms of GHE on plasma and hepatic lipid metabolisms in high-fat (HF) diet-fed rats. Rats were divided into: normal diet group, HF-diet group, HF-dietþ5% GHE group, and HF-dietþ1% cholestyramine group. GHE supplementation for 8 weeks significantly decreased plasma cholesterol, LDL-C, and VLDL-C levels and increased the fecal triglyceride and bile acid excretion in HF diet-fed rats. GHE group has lower lipid contents in the liver and adipose tissues. GHE supplementation decreased the activities of acetyl-CoA carboxylase, fatty acid synthase, and HMG-CoA reductase in the livers. The levels of increased phosphorylated AMP-activated protein kinase (AMPK), peroxisome proliferator activated receptor (PPAR)-a, farnesoid-X receptor (FXR), low density lipoprotein receptor (LDLR), and cytochrome P450-7A1 (CYP7A1) protein expression, and the decreased PPAR-g protein expression in the livers were observed in GHE group. These results suggest that GHE supplementation is capable of interfering in cholesterol metabolism and increasing hepatic LDLR and CYP7A1 expression to decrease blood cholesterol, and activating FXR and AMPK to inhibit lipogenic enzyme activities and reduce the hepatic lipid accumulation.

Keywords: Cholestyramine, High-fat diet-fed rats, Lipid metabolism, Polysaccharide-rich Gelidium amansii hot-water extract

https://doi.org/10.38212/2224-6614.1181

(https://www.jfda-online.com/journal/vol29/iss1/4)
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