Determination of Simazine Residue in Sugarcane by Application of Matrix Solid Phase Dispersion (MSPD) Extraction Technique
SU-HSIANG TSENG1,2, YU-JU LIN1, PI-CHIOU CHANG1, SHIN-SHOU CHOU1* AND HUNG-MIN CHANG2
1. Bureau of Food and Drug Analysis, Department of Health, Executive Yuan, Taipei 115, Taiwan (R.O.C.)
2. Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 106, Taiwan (R.O.C.)
(Received: June 20, 2003; Accepted: September 16, 2003)
ABSTRACT
A rapid method for the determination of simazine residue in sugarcane by matrix solid phase dispersion (MSPD) extraction and florisil cleanup was developed. This method performed extraction and cleanup in a single eluting step and significantly reduced organic solvent consumption. Two grams of homogenized sample was blended with 2 g of EnvirElut sorbent until a homogeneous mixture was observed. The mixture was transferred into a florisil cartridge, and eluted with 25 mL of dichloromethane. The eluate was evaporated to dryness, and the residue was dissolved with acetonitrile and then determined by HPLC-UV at 230 nm. Recoveries of simazine spiked in sugarcane (0.1~0.5 ppm) were determined to be 86.9~94.7%, while coefficient of variation was determined to be less than 10%. The detection limit was 0.02 ppm.
Key words: pesticide residue, sugarcane, matrix solid phase dispersion, simazine, high performance liquid chromatography