Isolation of GLP-1 enhancing indolizidine alkaloids from Boehmeria formosana

Ching-Yi Lu,^a Zheng-Ying Huang,^a Meng-Chin Chen,^a Hui-Kang Liu,^b Shoei-Sheng Lee,^a,* Chia-Chuan Chang^a,*

^a School of Pharmacy, College of Medicine, National Taiwan University, Taipei 10050, Taiwan, R.O.C.

^b National Research Institute of Chinese Medicine, Taipei 11221, Taiwan, R.O.C.

Boehmeria formosana, with its related species, demonstrates anti-glycemic effect, inhibition of HBV production, anti-cancer activities, etc. Some indolizidine alkaloids from the same genus are bioactive but sensitive to light. To overcome this problem and obtain more phenanthroindolizidine alkaloids, isolation was performed in darkness, yielding 10 new indolizidine alkaloids and 17 known compounds. Among them, seven enhanced glucagon-like receptor 1 (GLP-1) activity at 50 mM, especially 14 and 6 (3.5- and 2.3-fold than the negative control). This procedure yielded bioactive indolizidine alkaloids with novel structures.

Keywords: Boehmeria formosana, secondary metabolites, phenanthroindolizidine alkaloids, 𝛼-glucosidase inhibitor, GLP-1-enhancing ability

https://doi.org/10.38212/2224-6614.3483