Jinhua Qinggan Granule UHPLC-Q-extractive-Orbitrap-MS assay: Putative identification of 45 potential anti-Covid-19 constituents, confidential addition, and pharmacopoeia quality-markers recommendation

Jingyuan Zeng ^a, Xican Li ^a,*, Rongxin Cai ^a,b, Chunhou Li ^a, Shaoman Chen ^a

^a School of Chinese Herbal Medicines, Guangzhou University of Chinese Medicine, Guangzhou, 510006, People's Republic of China

^b College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, People's Republic of China

The study combined UHPLC-Q-Orbitrap-MS analysis with authentic standards, to create a novel strategy for isomers recognition and putative identification. Through the strategy, anti-Covid-19 Jinhua Qinggan Granule was found to comprise 28 isomers and 45 potential anti-Covid-19 constituents. The detection of three constituents (Danshensu, cryptotanshin, and tanshinone IIA) suggests Danshen as confidential additive. Based on this, 6 constituents are recommended as quality-marker candidates, including chlorogenic acid, acteoside, peimisine, baicalein, licoricesaponin H2, and tanshinone IIA. Obviously, the study can not only help the public to really understand the Granule's formula and chemistry, but also facilitate its Pharmacopoeia collection in future.

Keywords: Isomer recognition, Pharmacopoeia collection, Q-marker, Quality-control, Three Chinese medicines and three Chinese recipes

https://doi.org/10.38212/2224-6614.3466