Quantification of anthocyanosides in grapes by QuEChERS and biphenyl-UHPLC tandem mass spectrometry

Lun-Chi Yang, Sung-Fang Chen^*

Department of Chemistry, National Taiwan Normal University, Taipei 11677, Taiwan

In this study, the QuEChERS and UHPLC-MS/MS method using a biphenyl stationary phase was developed and applied for the rapid separation of 12 anthocyanosides in grapes. Efficient separation was achieved for a wide range of mono- and un-glycosylated anthocyanosides. The linear ranges of the target anthocyanosides were 0.5 to 500 ng mL⁻¹. The intra- and inter-day precisions were both below 11.8%. Anthocyanosides in grapes from different sources were successfully quantified. Thus, the developed QuEChERS and UHPLC-MS/MS method provide an attractive alternative for the quantification of anthocyanosides in grapes.

Keywords: Anthocyanosides; Biphenyl stationary phase; LC-MS/MS; QuEChERS; Quantification

https://doi.org/10.38212/2224-6614.3427