Chemiluminescence of lucigenin-tetracycline and itsapplication for sensitive determination of procyanidin

Shuai Quan ^a,b, Kaixiang Ji ^a,b, Fangshuo Liu ^b,c, Tesfaye Hailemariam Barkae ^b,c, Mohamed Ibrahim Halawa ^d,e, Saima Hanif ^f, Baohua Lou ^b,c,*,Jianping Li ^a,**, Guobao Xu ^a,b,c,***

a College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China

b State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China

c University of Science and Technology of China, Hefei, 230026, China

d Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt

e Guangdong Laboratory of Artificial Intelligence & Digital Economy (SZ), Shenzhen 518060, PR China

f Department of Biological Sciences, National University of Medical Sciences (NUMS), The Mall, Abid Majeed Road Rawalpindi, Punjab,46000, Pakistan

Tetracycline was reported to trigger intense lucigenin chemiluminescence (CL). Based on its significant quenching to lucigenin-tetracycline CL, procyanidin was detected. The mechanism of the lucigenin-tetracycline CL and its quenching by procyanidin were proposed. Under the optimum conditions, the calibration curve is linear in the range of 10-1000 nM for procyanidin with a detection limit of 4.4 nM. The RSD for nine replicate determinations of procyanidin was less than 1.7%. Moreover, this method was satisfactorily applied to the determination of procyanidin in human urine samples and health food capsule of grape seed.

Keywords: Chemiluminescence, Lucigenin, Procyanidin, Tetracycline

https://doi.org/10.38212/2224-6614.3411