A three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma

Cheng-Wei Cheng ^a, Wen-Han Feng ^b, Wei-Hua Tang ^c, Hwang-Shang Kou ^a, Chun-Chi Wang ^a,d,e,*, Shou-Mei Wu ^f,g,**

a School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC

b Department of Internal Medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University Hospital, Kaohsiung

Medical University, Kaohsiung, Taiwan, ROC

c Department of Internal Medicine, Taipei Veterans General Hospital Yuli Branch, Hualian, Taiwan, ROC

d Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan, ROC

e Drug Development and Value Creation Research Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan, ROC

f Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC

g Taiwan Food and Drug Administration, Ministry of Health and Welfare, Taipei, Taiwan, ROC

A three-step stacking capillary electrophoresis (CE) composed of field-amplified sample injection, sweeping, and analyte focusing by micellar collapse (FASI-sweeping-AFMC) was developed to determine dabigatran (D) and its major active metabolite, dabigatran acyl-beta-d-glucuronide (DAG), in human plasma. After optimization and validation, this novel approach was further applied to monitor 5 real samples, and the 25.2 to 186.8 ng mL^-1 D could be observed among those. Based on these results, the novel CE stacking strategy was successfully applied for the analysis of D and DAG in human plasma and could be served as a tool for clinical assays.

Keywords: Anticoagulant, Dabigatran, Dabigatran acyl-beta-d-glucuronide, FASI-sweeping-AFMC, Human plasma

https://doi.org/10.38212/2224-6614.3391