Lucigenin-pyrogallol chemiluminescence for the multiple detection of pyrogallol, cobalt ion, and tyrosinase

Islam M. Mostafa ^a,b,c, Muhammad Rehan Hasan Shah Gilani ^a,d, Yequan Chen ^e,

Baohua Lou ^a,b,*, Jianping Li ^e,*, Guobao Xu ^a,b,*

a State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences,

Changchun, Jilin 130022, PR China

b University of Science and Technology of China, Hefei, 230000, PR China

c Department of Analytical Chemistry, Faculty of Pharmacy, Minia University, 61519, Minia, Egypt

d Institute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan

e College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China

Developing new, cheap, sensitive and selective chemiluminescence (CL) systems with multiple detection properties is still a big challenge for biological and environmental applications. Here, we report a new CL system having multiple detection applications (environmental and biological). The developed lucigenin-pyrogallol system gave an enhancement (190 times) over the conventional lucigenin-H2O2 CL system. Cobalt ion and tyrosinase can inhibit lucigenin-PG CL intensity.‎ Based on these phenomena, we have developed new CL methods for the detection of pyrogallol (LOD=0.94 µM), lucigenin (LOD=0.42 µM), cobalt (LOD=68 nM), and tyrosinase (LOD=1.8 µg/mL). Furthermore, the developed system gave excellent recoveries in real samples within the range of 93.59−103.12% for the assay of pyrogallol, Co2+ and tyrosinase in coffee, lake water, and human plasma, respectively.

Keywords: Chemiluminescence, Cobalt, Lucigenin, Pyrogallol, Tyrosinase

https://doi.org/10.38212/2224-6614.3361

(https://www.jfda-online.com/journal/vol29/iss3/9)