Multiclass analysis of antimicrobial drugs in shrimp muscle by ultra high performance liquid chromatography-tandem mass spectrometry
Saksit Susakate a, Saranya Poapolathep a, Chanat Chokejaroenrat b, Phanwimol Tanhan a, Jana Hajslova c, Mario Giorgi d, Kanista Saimek e, Zhaowei Zhang f, Amnart Poapolathep a,*
a Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand
b Department of Environmental Technology and Management, Faculty of Environment, Kasetsart University, Bangkok 10900, Thailand
c Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague 6-Dejvice, Czech Republic
d Department of Veterinary Sciences, University of Pisa, Via Livornese, San Piero a Grado, 56122, Pisa, Italy
e Department of Laboratory, Charoen Pokphand Foods Public Company Limited, Klang, Rayong 21170, Thailand
f Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
A reliable, selective and rapid multiclass method has been developed for the simultaneous determination of 55 antibacterial drug residues in shrimp muscle samples by ultra high performance liquid chromatography-tandem mass spectrometry. The investigated compounds comprise of eight different classes, namely fluoroquinolones, sulfonamides and synergistic agents, tetracyclines, macrolides, lincosamides, penicillins, nitroimidazole and amphenicols. A simple liquid extraction procedure was developed consisting of extraction with a mixture of acetonitrile and ethylenediaminetetraacetic acid (EDTA), followed by a defatting step with n-hexane. Chromatographic conditions were optimized, obtaining a running time <10 min. Mean recoveries ranged from 74.3% to 113.3%. For precision test, relative standard deviations (RSD, %) were lower than 15.0% and 24.0% for repeatability and reproducibility, respectively. Limits of detection and quantification ranged from 1.0 to 5.0 ng/g and 3.0e10.0 ng/g, respectively. Finally, the method was applied to real samples and the results demonstrated that enrofloxacin, ciprofloxacin, pefloxacin and doxycycline were quantifiable in shrimp samples.
Keywords: Antimicrobial drugs, Multiclass analysis, Shrimp, UHPLC-MS/MS