Quantitative and qualitative analyses of cytotoxic triterpenoids in the rhizomes of Anemone raddeana using HPLC and HPLC-ESI-Q/TOF-MS

Ying Zhao, Xin Zhang, Chongning Lv, Yang Yu, Yu Zhang, Jincai Lu^*

School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103 wenhua Road, Shenyang, 110016, China

Anemone raddeana Regel, a Traditional Chinese Medicine, has been demonstrated to possess cytotoxicity and anti-inflammatory activities. The purpose of this study is to establish analytical methods to identify and quantify the major active constituents in Anemone raddeana. A high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (HPLC-ESI-Q/TOF-MS) was used to identify the components in the title plant material. To quantify the major components, a HPLC-UV method was developed and validated. The results showed that 37 compounds were identified based on the MS data and retention times. The contents of eight main bioactive compounds were determined by HPLC simultaneously. These methods could be used to effectively evaluate the quality of A. raddeana and provide a valuable reference for further study. In addition, the cytotoxicity activity of the different fractions of A. raddeana was determined. Hederacolchiside A1 (f) showed promising activity against ten human cancer cell lines with IC50 values from 0.29 to 3.48 mM.

Keywords: Anemone raddeana Regel, Quantification, Cytotoxicity