Phosphorylation and antiaging activity of polysaccharide from Trichosanthes peel

Min Zhang ^a,1, Nana Su ^a,1, Qianli Huang ^b, Qiang Zhang ^a,c, Yufen Wang ^a, Jinglei Li ^a,*, Ming Ye ^a,*

^a School of Food Science and Engineering, Hefei University of Technology, Hefei, China

^b School of Biological and Medical Engineering, Hefei University of Technology, Hefei, China

^c College of Biological Science and Engineering, Beifang University of Nationalities, Yinchuan, China

Polysaccharides from Trichosanthes peel (TPP) were obtained by ultrasound-assisted extraction. TPP-1 was separated from the TPP by Sephadex G-100 column chromatography. Phosphorylation of TPP-1 was carried out and phosphorylated TPP-1 was named as PTTP-1. The results of infrared spectra, 13C nuclear magnetic resonance spectra and 31P nuclear magnetic resonance spectra showed that the main structure of PTPP-1 was similar to that of TPP-1 and -H2PO3 groups which were conjugated to C-6 of →4)-α-D-Manp-(1→, C-4 of →6)-α-D-Galp-(1→, C-2 and C-3 of →1)-α-L-Araf, C-2 of →1)-α-L-Araf-(3→, and C-6 and C-3 of →1)-α-D-Glcp. In vivo antiaging activity results proved that TTP-1 and PTTP-1 could both significantly improve the body weight, spleen index, and thymus index of the D-galactose-induced aging mice, increase the levels of superoxide dismutase, catalase, glutathione peroxidase, and reduce malondialdehyde contents in the liver, brain, and serum of aging mice. These results indicated that both TPP-1 and PTTP-1 presented significant antiaging activity. Moreover, PTTP-1 showed stronger antiaging effects in aging mice, indicating that phosphorylation improved antiaging effect.

Keywords: antiaging activity, phosphorylation modification, Trichosanthes peel polysaccharide