Optimization conditions for extracting polysaccharide from Angelica sinensis and its antioxidant activities
Suyang Tian a,b, Changchun Hao a,*, Guangkuan Xu a, Juanjuan Yang a,Runguang Sun a,*
a Laboratory of Biophysics and Biomedical Engineering, School of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China
b Lintong Middle School, Xi'an 710600, China
In this study, polysaccharides from Angelica sinensis were extracted using the ultrasoundassisted extraction method. Based on the results of single factor experiments and orthogonal tests, three independent variablesdwater/raw material ratio, ultrasound time, and ultrasound powerdwere selected for investigation. Then, we used response surface methodology to optimize the extraction conditions. The experimental data were fitted to a quadratic equation using multiple regression analysis, and the optimal conditions were as follows: water/raw material ratio, 43.31 mL/g; ultrasonic time, 28.06 minutes; power, 396.83 W. Under such conditions, the polysaccharide yield was 21.89 ± 0.21%, which was well matched with the predicted yield. In vitro assays, scavenging activity of superoxide
anion radicals, hydroxyl radicals, and 2,2-diphenyl-1-picry-hydrazyl radical showed that polysaccharides had certain antioxidant activities and that hydroxyl radicals have a remarkable scavenging capability. Therefore, these studies provide reference for further research and rational development of A. sinensis polysaccharide.