Identification of cow milk in goat milk by nonlinear chemical fingerprint technique
Yong-Jie Ma a,b,*, Wen-Bin Dong b, Cheng Fan c, Er-Dan Wang d
a College of Food and Drug, Luoyang Normal University, Luoyang 471934, China
b College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, Xi'an 710021,
China
c Institute of Product Quality Supervision and Inspection, Shaanxi Province, Xi'an 710048, China
d College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
The objective of this paper was to develop a nonlinear chemical fingerprint technique for identifying and detecting adulteration of goat milk with cow milk. In this study, by taking the Belousov-Zhabotinsky oscillatory chemical reaction using acetone and substrates in goat milk or cow milk as main dissipative substances, when the same dosage of goat milk and cow milk was introduced to the “Hþ þ Mn2þ þ BrO3 þ acetone” oscillating system
respectively, nonlinear chemical fingerprints were obtained for goat milk and cow milk from the same origin. The results showed that inductive time value and the content of cow milk in goat milk had a linear relationship in the range of 0e100% and the corresponding regression coefficient was 0.9991. A detection limit of 0.0107 g/g was obtained, and the content of cow milk in mixed milk was calculated. The proposed method in this study was simple, economical and effective. In addition, the method did not need the pretreatment and separation of samples for identifying and evaluating cow milk adulteration in goat milk.