Characterization of nine polyphenols in fruits of Malus pumila Mill by high-performance liquid chromatography

 

Lu Bai ^a,b, Sen Guo ^a,b, Qingchao Liu ^a,b, Xueqin Cui ^a,b, Xinxin Zhang ^a,b,
Li Zhang ^a,b, Xinwen Yang ^c, Manwei Hou ^c, Chi-Tang Ho ^d, Naisheng Bai a,b,*

 

^a College of Chemical Engineering, Northwest University, Xi'an, Shaanxi, China
^b Department of Pharmaceutical Engineering, Northwest University, Xi'an, Shaanxi, China
^c Shaanxi Province Apple Industry Research and Development Center, Xi'an, Shaanxi, China
^d Department of Food Science, Rutgers University, New Brunswick, NJ, USA

 

Polyphenols are important bioactive substances in apple. To explore the profiles of the nine representative polyphenols in this fruit, a high-performance liquid chromatography method has been established and validated. The validated method was successfully applied for the simultaneous characterization and quantification of these nine apple polyphenols in 11 apple extracts, which were obtained from six cultivars from Shaanxi Province, China. The results showed that only abscission of the Fuji apple sample was rich in the nine apple polyphenols, and the polyphenol contents of other samples varied. Although all the samples were collected in the same region, the contents of nine polyphenols were different. The proposed method could serve as a prerequisite for quality control of Malus products.

 

Keywords: high-performance liquid chromatography method, Malus pumila Mill, phloridzin, polyphenols, quantitative analysis