Simultaneous Determination of Morphine HCl, Ketamine HCl and Droperidol in 0.9% Sodium Chloride by HPLC
DEREK K. T. LEE1, ANDY H. J. CHIOU2 AND DA-PENG WANG3*
1. Department of Pharmacy, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C.
2. Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, R.O.C.
3.School of Pharmacy, National Defense Medical Center, Taipei, Taiwan, R.O.C.
(Received: August 3, 2004; Accepted: December 31, 2004)
ABSTRACT
A simple and rapid liquid chromatographic method is presented for the determination of morphine HCl, ketamine HCl and droperidol in 0.9% sodium chloride injection stored in polyvinyl chloride (PVC) infusion bags. Assay was performed on a pre-packed HPLC Hypersil BDS C18 3.9 mm × 15 cm, particle size 3 μm column under controlled ambient temperature. Separation among morphine HCl, ketamine HCl, droperidol and their associated degradation compounds was achieved by isocratic elution using mobile phase consisting of methanol/acetonitrile/0.01 M 1-octane sulfonic acid sodium in 0.005 M phosphate buffer (pH 4.0) (50/50/100) at a flow rate of 1.0 ml/min. An UV/VIS variable programmable wavelength detector set at 254 nm was used. Sample volumes of 20 μL were
injected. There was no need for sample pre-treatment.
Key words: morphine HCl, ketamine HCl, droperidol, 0.9% sodium chloride, HPLC