Determination of Patulin in Apple Juice Produced in Isparta, Turkey by HPLC with Diode Array Detection
A. HAKAN AKTA 1*, MUSTAFA YILMAZER1 AND AHINDE DEM RC2
1. Department of Chemistry, Art Science Faculty, Suleyman Demirel University, 32210 Isparta, Turkey
2. Department of Chemistry, Faculty of Science, METU, Ankara, Turkey
(Received: October 31, 2003; Accepted: May 18, 2004)
ABSTRACT
In this study patulin was determined by HPLC on a reversed phase C18 column with UV detection at 276 nm. Patulin was extracted with ethyl acetate and then was cleaned up by extraction with sodium carbonate solution. The mobile phase and the column used in the HPLC determination were selected to separate patulin from the interfering substances in apple juice, especially 5-hydroxymethylfurfural (HMF). The linear regression coefficient of the standard curve (y = 327x – 292) for patulin at concentrations ranging from 15 to 200 μg/L was 0.9998 (N = 6). The results were corrected for the recovery percentage. The method is rapid and convenient for the determination of patulin in apple juice.
Key words: patulin, HPLC, fruit juice