HPLC/Colorimetry Combination Method for Quantitative Analyses of Sclerotiorin Produced by Penicillium
sclerotiorum
SHUN-HSIANG WENG1,2, NAN-WEI SU1, YOUK-MENG CHOONG2 AND MIN-HSIUNG LEE1*
1. Graduate Institute of Agricultural Chemistry, National Taiwan University, 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan, R.O.C.
2. Department of Food Science and Technology, Tajen Institute of Technology, 20 Weisin Rd. Yanpu Township, Pintung County 907, Taiwan, R.O.C.
(Received: October 16, 2003; Accepted: December 22, 2003)
ABSTRACT
In this study, quantitative analysis of sclerotiorin from Penicillium sclerotiorum was established by using HPLC/colorimetry combination method at the condition of unavailability of authentic sclerotiorin compound. Mycelium was extracted with methanol, then analyzed by HPLC (a C18 reverse phase column with acetonitrile/water = 65/35, v/v as mobile phase and detected at 370 nm) and simultaneously measured OD370 with spectrophotometers. The limit of quantitation (LOQ) was 0.5 μg/mL. Recovery studies were performed using the lyophilized mycelia, with each spiked with sclerotiorin at 1 and 10 mg, respectively. The recoveries were found in the range of 97~109%. The coefficients of variation were less than 3.8% with this HPLC / OD370 combination method.
Key words: Penicillium sclerotiorum, sclerotiorin, HPLC/colorimetry combination method, quantitative analyses