Applications of Polyion-sensitive Electrodes to Pharmaceutical Domain: a Mini-review
AN-RONG LEE 1*, LI-CHIEN CHANG 1, VICTOR C. YANG 2, LI-HENG PAO 1 AND DA-PENG WANG 1
1.School of Pharmacy, National Defense Medical Center, 161 Min-Chuan E. Road, Sec. 6, Taipei 114, Taiwan
2. College of Pharmacy, The University of Michigan, Ann Arbor, U.S.A.
(Received: August 05, 2002; Accepted: October 14, 2002)
ABSTRACT
Recent development in the pharmaceutical applications of polyion-sensitive electrodes (PSEs) is reviewed. The general electrochemical principle governing the potentiometric response of such polymer membrane-based devices is summarized and applications for the use of these novel sensors are detailed. These new applications include methods to quantitate the anticoagulant heparin and pentosan polysulfate, an anti-osteoarthritis drug, via titration with polycationic protamine or polyarginine, as well as selective assay on fibrinolytic enzymes (e.g., plasminogen activators and plasminogen) using protamine as the substrate based on potentiometric polyion detection.
Keywords: biosensor, polyion determination, pharmaceutical applications, heparin, pentosan polysulfate, fibrinolytic enzymes.