Determination of 24 sulfonamide antibiotics in instant pastries by modified QuEChERS coupled with ultra performance liquid chromatography-tandem mass spectrometry

Shuo Li ^a, Can Zhang ^b, Hui-Xin Tang ^a, Yue Gu ^a, Ai-Jing Guo ^a, Ke Wang ^a,*, Kao-Qi Lian ^b,c,**

^a Shijiazhuang Technology Innovation Center for Chemical Poison Detection and Risk Early Warning, Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang 050011, China

^b School of Public Health, Hebei Medical University, Shijiazhuang, 050017, China

^c Hebei Key Laboratory of Environment and Human Health, Hebei Medical University, Shijiazhuang, 050017, China

There were few reports about antibiotic residues in egg-containing products. In the study, an effective method for the simultaneous determination of 24 sulfonamide antibiotics in two instant pastries based on a modified QuEChERS sample preparation technique coupled with ultra performance liquid chromatography-tandem mass spectrometry was developed. The results show that the average recoveries of the SAs at 5, 10, and 50 μg kg^-1 levels were 67.6%-103.8%, with relative standard deviations (RSD) of 0.80-9.23%. The limit of detections (LODs) and limit of quantitations (LOQs) were 0.01-0.14 μg kg^-1 and 0.02-0.45 μg kg^-1, respectively. This method was suitable for analysis of 24 SAs in instant pastries.

Keywords: Instant pastries, QuEChERS, Sulfonamide antibiotics, Ultra performance liquid chromatography-tandem mass spectrometry

https://doi.org/10.38212/2224-6614.3434

(https://www.jfda-online.com/journal/vol31/iss1/5/)