Non-digestive Stachyose Enhances Bioavailability of Isoflflavones for Improving Hyperlipidemia and Hyperglycemia in Mice Fed with High Fat Diet

Yingmei Wu ^a,b, Yalong Lu ^a, Daoyuan Ren ^a,*, Xuefeng Chen ^a, Xingbin Yang ^a,**

a Shaanxi Engineering Laboratory for Food Green Processing and Safety Control, and Shaanxi Key Laboratory for Hazard Factors Assessment in Processing and Storage of Agricultural Products, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, 710119, China

b College of Biology and Food Engineering, Chongqing Three Gorges University, Chongqing, 404120, China

This study examined the efficacy of non-digestive stachyose on enhancing the absorption of soy isoflavones to improve metabolic syndrome in C57/BL6 mice. UPLC-q/TOF-MS was employed to analyze the content of isoflavones in urine and faeces. Stachyose significantly increased urinary contents of total isoflavones, genistein, daidzein and glycitein in mice. Supplementation of stachyose, soybean isoflavones or a combination prevented high fat diet (HFD)-induced body weight gain, accumulated adipose, dyslipidemia and hyperglycemia in obese mice. Interestingly, co-supplementation of stachyose and isoflavones improved all the mentioned parameters more effectively than administration of stachyose or isoflavones alone. Histological observation of hepatic tissues also confirmed the beneficial effects of co-supplementation of stachyose and isoflavones. These findings suggest that co-ingestion of non-digestible oligosaccharides and polyphenols as normal diet is a promising potential strategy for managing or reducing the risk of metabolic syndrome, which will lead to new knowledge on whole soybean and have extensive application in development of healthy food.

Keywords: Enhancing absorption, Hyperlipidemia, Hyperglycemia, Soybean isoflavones, Stachyose

https://doi.org/10.38212/2224-6614.3078

(https://www.jfda-online.com/journal/vol29/iss1/7)