Voltammetric Quantification of Fluoxetine: Application to Quality Control and Quality Assurance Processes
RUI P. LENCASTRE1, CRISTINA D. MATOS1, JORGE GARRIDO1, FERNANDA BORGES2 AND E. MANUELA GARRIDO1*
1. Department of Chemical Engineering, School of Engineering ISEP, Polytechnic Institute of Porto, Rua S. Tomé, 4200-485 Porto, Portugal.
2. Research Unit “Molecular Physical-Chemistry”, Department of Organic Chemistry, Faculty of Pharmacy,
University of Porto, 4050-047 Porto, Portugal.
(Received: February 20, 2006; Accepted: June 1, 2006)
ABSTRACT
(Received: February 20, 2006; Accepted: June 1, 2006)
The oxidative behaviour of fluoxetine was studied at a glassy carbon electrode in various buffer systems and at different pH using cyclic, differential pulse and square wave voltammetry. A new square wave voltammetric method suitable for the quality control of fluoxetine in commercial formulations has been developed using a borate pH 9 buffer solution as supporting electrolyte. Under optimized conditions, a linear response was obtained in the range 10 to 16 μM with a detection limit of 1.0 μM. Validation parameters such as sensitivity, precision and accuracy were evaluated. The proposed method was successfully applied to the determination of fluoxetine in pharmaceutical formulations. The results were statistically compared with those obtained by the reference high-performance liquid chromatographic method. No significant differences were found between the methods.
Key words: Fluoxetine, square wave voltammetry, oxidation, quality control, pharmaceutics.