Determination of Glucosamine Content in Nutraceuticals by Capillary Electrophoresis Using In-Capillary OPA Labeling Techniques
Jong-Jyh Chen, Yeun Chung Lee, Tzong-Jih Cheng, Hsien-Yi Hsiao and Richie L. C. Chen*
Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan, R.O.C.
(Received: August 22, 2005; Accepted: March 21, 2006)
Capillary electrophoresis (CE) procedure was developed for determining glucosamine content in nutraceuticals. Glucosamine was labeled with o-phthalaldehyde (OPA) by mixing with the OPA reagent (4.5 mM OPA, 4.5 mM 2-mercaptoethanol dissolved in 20 mM borate buffer, pH 9.3). The fluorescent OPA-glucosamine adduct was separated and detected at A340 within 3 min. The pre-capillary labeling reaction can be performed by sequentially injecting OPA/sample/OPA segments into the separation capillary; the in-capillary labeling technique simplified the sample pretreatment and improved its reproducibility. The linear dynamic range of the in-capillary labeling CE method was 0.1~30 mM. No sample pretreatment procedure was required for tablets containing mainly starch or other sugar as the vehicles.
Key words: capillary electrophoresis, glucosamine, nutraceutical, in-capillary labeling, o-Phthalaldehyde