High Performance Liquid Chromatography to Determine Animal Drug Clenbuterol in Pork, Beef and Hog Liver
LIN-YING CHANG1,2, SHIN-SHOU CHOU1* AND DENG-FWU HWANG3
1. Bureau of Food and Drug Analysis, Department of Health, Executive Yuan, 161-2 Kunyang St., Nangang District, Taipei City 115, Taiwan, R.O.C.
2. Graduate Institute of Applied Science of Living, Chinese Culture University, 55 Hwa-Kang Rd., Yang-Ming-Shan, Taipei City 111, Taiwan, R.O.C.
3. Department of Food Science, National Taiwan Ocean University, 2 Pei-Ning Rd., Jhongjheng District, Keelung City 202, Taiwan, R.O.C.
(Received: November 4, 2004; Accepted: February 24, 2005)
ABSTRACT
A semi-micro high performance liquid chromatography (HPLC) was developed to determine clenbuterol in pork, beef and hog liver. The procedure included extraction with 0.4 N perchloric acid, partitionally separation with diethyl ether, and then back-extraction with 0.2 M sulphuric acid. Analytical procedure was performed by HPLC with a Waters Cosmosil column 5C18-MS (2.0 × 150 mm), a mobile phase of 0.05 M NaH2PO4 (pH3.0)/acetonitrile solution (80/20, v/v) and absorbance at 212 nm. The recoveries of clenbuterol in pork, beef and hog liver spiked with standards of 0.0005~0.01 ppm were 80.9~90.6 %. The detection limit of clenbuterol in tested
samples was 0.0001 ppm, which is lower than the officially allowed level. The equation was linear for detecting 0.0002~0.001 ppm clenbuterol. The method was validated to be usable for market samples. Three out of 150 samples were detected to contain clenbuterol (0.0001~0.00015 ppm), which were less than both JECFA and Department of Health regulation levels (hog liver 0.0006 ppm and beef 0.0002 ppm).
Key words: clenbuterol, semi-micro HPLC, pork, beef, hog liver