Determination of a-Hydroxyacids in Cosmetics
WEI-SHENG HUANG, CHENG-CHIN LIN, MING-CHUAN HUANG AND KUO-CHING WEN*
National Laboratories of Foods and Drugs, Department of Health, Executive Yuan, 161-2, Kuen Yang Street, Nankang, Taipei, Taiwan, R.O.C.
(Received: April 23, 2001; Accepted: November 1, 2001)
ABSTRACT
A high performance liquid chromatographic method was developed for the simultaneous determination of four a-hydroxyacids (AHAs, Glycolic acid, dl-Malic acid, Lactic acid and Citric acid) in cosmetics. Samples were analyzed using a reverse-phase C18 column (Capcell PAK C18 UG120 S-5 mm) with 2% phosphoric acid (pH 2.0) as mobile phase at 210 nm. Calibration curves of four a-hydroxyacids were constructed in the range of 50-500 mg/mL and their correlation coefficients were in the range of 0.9992-0.9995. The relative standard deviations of four a-hydroxyacids for intraday and interday analyses were 0.05~1.49% and 0.72~3.24%, respectively. The average recoveries of four a-hydroxyacids ranged from 96.3% to 99.2%.
Keywords: high performance liquid chromatography, a-hydroxyacids, AHAs