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9. Fluorescent carbon nanodot-based rapid cell nucleus staining and application for diagnosis
| 發布日期:2024-06-12 | 更新日期: 發布單位:

Fluorescent carbon nanodot-based rapid cell nucleus staining and application for diagnosis

Daiming Zhong a, Huamin Yuan a, Qiuyan Tian a, Gongping Deng b, Yanhong Ouyang b, Yi Chen c, Yang Wang b, Zhiqin Yuan d,*

a Laboratory Medicine Department, Hunan Prevention and Treatment Institute for Occupational Diseases, Changsha 410000, Hunan,

China

b Department of Emergency, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou 570311,

Hainan, China

c Hunan Zhixiangweilai Biotechnology Co., Ltd, Changsha 410125, Hunan, China

d College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China

Cell nucleus status decides the activities of corresponding cells, making its rapid and effective staining important for revealing the actual condition of biological environment in life science and related fields. In this study, fast staining of cell nucleus is realized by fluorescent carbon nanodots (CDs). The staining mechanism is due to the positively charged CD surface-induced cell membrane penetration, which facilitates the CD-nucleus binding via electrostatic attraction. The size of cell nucleus is easily measured with fluorescence imaging technique. In addition, the CD-based cell nucleus stain is applied for discriminating the normal and cancer cells by determining the cell-to-nucleus ratio with fluorescence images.

Keywords: Cancer diagnosis, Carbon nanodots, Cell nucleus, Fluorescence imaging, Staining

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