Analysis of Antiretroviral Drugs in Biological Matrices for Therapeutic Drug Monitoring
Armağan Önal
Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, Istanbul 34116, Turkey
(Received: July 5, 2005; Accepted: January 19, 2006)
Abstract
High-performance liquid chromatographic (HPLC) analysis of antiretroviral (ARV) drugs in biological matrices are reviewed for therapeutic drug monitoring purposes. HPLC is the most often used analysis method of these drugs. Liquid-liquid extraction, solid phase extraction and protein precipitation were used for the prepurification of the biological samples. ARV drugs were detected by various detection methods, including ultraviolet, fluorescence, mass spectrometry (MS) and MS/MS. This review shows that HPLC methods allow quantitative determination of antiretroviral drugs, individually or simultaneously in biological matrices for therapeutic drug monitoring purposes.
Key words: therapeutic drug monitoring, protease inhibitors, nucleoside reverse transcriptase inhibitors, nucleotide reverse transcriptase inhibitors, high performance liquid chromatography