Molecular cartography of residue pesticides on grape surface in 3D by ambient ionization tandem mass spectrometry

Hung Su ^a,1, Tiao-Lai Huang ^b,c,1, Cheng-Ting Chi ^a, Yi-Tzu Cho ^d, Chi-Wei Lee ^e,***, Jingyueh Jeng ^f,**, Jentaie Shiea ^a,g,h,*

a Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan

b Department of Psychiatry, Chang Gung Memorial Hospital-Kaohsiung Medical Center and Chang Gung University College of Medicine, Kaohsiung, Taiwan

c Genomic and Proteomic Core Laboratory, Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan

d Department of Cosmetic Applications and Management, Yuh-Ing Junior College of Health Care & Management, Kaohsiung, Taiwan

e Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung, Taiwan

f Department of Medicinal Chemistry, Chia Nan University of Pharmacy and Science, Tainan, Taiwan

g Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan

h Research Center for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

Thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) was used to characterize the residual pesticides that were collected from the surface of a grape with metallic sampling probes. Fungicides, insecticides, and miticides were detected, where results were validated by simple solvent extraction followed by gas chromatography tandem mass spectrometry and liquid chromatography tandem mass spectrometry analyses. To explore the distribution of pesticide residues on grape surfaces, 149 locations of a grape surface were collected and followed by TD-ESI/MS/MS analysis. The molecular cartography was then generated from analysis of residual pesticides on the grape surface in 3D.

Keywords: Grape, Molecular cartography, Residual pesticide, Sampling probe, Thermal desorption-electrospray ionization tandem mass spectrometry

https://doi.org/10.38212/2224-6614.3385

(https://www.jfda-online.com/journal/vol29/iss4/16)