Journal of Food and Drug Analysis (JFDA)
【Update Date:2022-09-15】unit:
Composition decipherment of Ficus pumila var. awkeotsang and its potential on COVID-19 symptom amelioration and in silico prediction of SARS-CoV-2 interference
Hao-Chun Hua,†, Szu-Yin Yub,†, Xiao-Shan Hunga, Chun-Han Suc,d, Yu-Liang Yangc,e, Chien-Kei Weia, Yuan-Bin Chengf, Yang-Chang Wuf,g,h, Chia-Hung Yena, Tsong-Long Hwangi,j,k, Shu-Li Chena, István Szatmáril, Attila Hunyadib, Yi-Hong Tsaia,m, **, Fang-Rong Changa,f,n,o,*
a Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
b Institute of Pharmacognosy, Interdisciplinary Excellence Centre, University of Szeged, 6720 Szeged, Hungary.
c Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan
d Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 333, Taiwan.
e Biotechnology Center in Southern Taiwan, Academia Sinica, Tainan 711, Taiwan
f Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
g Graduate Institute of Integrated Medicine, China Medical University, Taichung 40402, Taiwan.
h Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 40402, Taiwan.
i Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan.
j Research Center for Chinese Herbal Medicine, Research Center for Food and Cosmetic Safety, and Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 33303, Taiwan.
k Department of Anesthesiology, Chang Gung Memorial Hospital 33305 Taoyuan, Taiwan.
l Institute of Pharmaceutical Chemistry and ELKH-MTA-SZTE Stereochemistry Research Group, Hungarian Academy of Sciences, University of Szeged, Szeged, Hungary.
m Department of Pharmacy and Master Program, Collage of Pharmacy and Health Care, Tajen University, Pingtung County 90741, Taiwan.
n Drug Development and Value Creation Research Center, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
o Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
† These authors contributed equally
The jelly from achenes of Ficus pumila var. awkeotsang (FPAA) is a famous beverage ingredient in Taiwan. In this work, ficumarin (1), a new compound was obtained from its twigs (FPAT) and elucidated with comprehensive spectroscopic data. The biosynthetic origin was proposed from the p‐coumaroyl‐CoA pathway. Alloxanthoxyletin, betulinic acid, and catechin were identified as the major and active constituents responsible for relieving neutrophilic inflammation by FPAT. Among them, the most potent alloxanthoxyletin was found to interact with PRO350 and GLU377 of human INOSOX. Further, Nrf2 activating capacity of the FPAT fraction and its coumarins was confirmed. With the analysis of LC-MS/MS data and feature-based molecular networking, coumarins were found as the dominant and responsible components. Notably, alloxanthoxyletin increased Nrf2 expression by up to 816.8±58% due to the interacting with the VAL561, THR560 and VAL420 residues of 5FNQ protein. COVID-19 Docking Server simulation indicated that pyranocoumarins would promisingly interfere with the life cycle of SARS-CoV-2. FPAT was proven to exert anti-inflammatory activity on neutrophils and to activate Nrf2, and may likely be developed as a complementary supplement in the treatment of COVID-19 patients.
Keywords: Anti-neutrophilic inflammation; COVID-19; Ficus pumila var. awkeotsang; Global natural products social molecular networking (GNPS); Nrf2 antioxidative defense system
https://doi.org/10.38212/2224-6614.3419