Mesonosides A-H, Primeverose Derivatives from Mesona procumbens, Suppress Adipogenesis by Downregulating PPARγ and C/EBPα in 3T3-L1 Cells

Hung-Tse Huang ^a,b, Chia-Ching Liaw ^b,c, Chun-Tang Chiou ^b, Kung-Ta Lee ^a,**, Yao-Haur Kuo ^b,d,*

a Department of Biochemical Science and Technology, National Taiwan University, Taipei, 10617, Taiwan

b Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei, 11221, Taiwan

c Department of Biochemical Science and Technology, National Chiayi University, Chiayi, 60004, Taiwan

d Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung, 40447, Taiwan

Obesity is becoming a worldwide epidemic, especially in industrialized countries. We hereby report a methanolic extract of Mesona procumbens (known as Hsian-tsao in Taiwan) significantly inhibits lipid accumulation in 3T3-L1 adipocytes, and eight new primeverose derivatives, mesonosides A-H (1-8), were isolated from the methanolic extract of M. procumbens. Structural elucidation of 1-8 was established by spectroscopic methods, especially 2D NMR techniques (1H-1H COSY, HSQC, HMBC, and NOESY) and HRESIMS. Anti-obesity evaluation revealed that isolates 1-5, 7, and 8 showed inhibitory effects on lipid accumulation and protein levels of adipogenic transcription factor, PPARγ and C/EBPα in 3T3-L1 cells. Our study suggests that M. procumbens extract including new primeverose isolates may be potentially used as a natural source to ameliorate fat accumulation and even obesity.

Keywords: Anti-adipogenic, Lipid accumulation, Mesona procumbens, Mesonoside, PPARγ

https://doi.org/10.38212/2224-6614.3365

(https://www.jfda-online.com/journal/vol29/iss3/5)