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Effect of Sulfite-treated Daylily (Hemerocallis fulva L.) Flower on the Production of Nitric Oxide and DNA Damage in Macrophages

【Update Date：2007-10-10】unit：

Effect of Sulfite-treated Daylily (Hemerocallis fulva L.) Flower on the Production of Nitric Oxide and DNA Damage in Macrophages

HUI-YIN CHEN1, JUNG-YI BOR2, WEN-HUA HUANG2 AND GOW-CHIN YEN2*

1. Department of Food Science, Central Taiwan University of Science and Technology,
11, Putzu Lane, Peitun District, Taichung 406, Taiwan, R.O.C.
2. Department of Food Science and Biotechnology, National Chung Hsing University, 250, Kuokuang Rd. Taichung 402, Taiwan, R.O.C.

(Received: July 18, 2006; Accepted: November 4, 2006)

ABSTRACT

   The inhibitory effect of fresh or dried daylilies extract on the generation of nitric oxide, and the effects of sulfur dioxide in dried daylily on nitric oxide production and DNA damage in Raw 264.7 macrophages were examined.  The extracts from sulfite-treated and untreated dried daylily flowers exhibited stronger scavenging effects on nitric oxide production by sodium nitroprusside (SNP) than that of fresh flower.  Fresh and untreated dried daylily flowers exhibited strong inhibitory effect on nitric oxide induced by lipopolysaccharide (LPS) in macrophages, but the sulfite-treated dried daylily did not.  The scavenging effect of sulfite on nitric oxide production by SNP was dose-dependent, and sulfite also exhibited low inhibitory effect on nitric oxide induced by LPS in macrophages.  Sodium hydrogen sulfite also slightly induced DNA damage in macrophages.  Although sulfur dioxide in dried flowers is volatilized during heating, its residual adverse effects should still be concerned.

Key words: daylily flower, Hemerocallis fulva L., nitric oxide, sulfur dioxide, DNA damage