Journal of Food and Drug Analysis (JFDA)
【Update Date:2020-09-11】unit:
Effect of 6,7-dimethoxy-2,2-dimethyl-2H-chromene (agerarin) on the recovery of filaggrin expression through targeting of Janus kinases in the inflammatory skin
Sung Shin Ahn a,1, Young Han Lee a,b,1, Hyunjin Yeo a, Youngshim Lee c, Do Sik Min d,Yoongho Lim b,c, Soon Young Shin a,b,*
a Department of Biological Sciences, Sanghuh College of Life Sciences, Konkuk University, Seoul, Republic of Korea
b Cancer and Metabolism Institute, Konkuk University, Seoul, Republic of Korea
c Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul, Republic of Korea
d College of Pharmacy, Yonsei University, Incheon, Republic of Korea
Filaggrin (FLG) is a structural component of the stratum corneum that is essential for maintaining the barrier function of the skin and for the formation of natural moisturizing factors. 6,7-Dimethoxy-2,2-dimethyl-2H-chromene (Agerarin) is a bioactive compound derived from Ageratum houstonianum, a plant that is used as a traditional medicine to treat skin diseases. This study aimed to evaluate the effect of agerarin on skin inflammation in a dinitrochlorobenzene (DNCB)-induced atopic dermatitis mouse model. We found that the topical administration of agerarin ameliorates atopic dermatitis-like skin lesions. We also showed that agerarin restores the reduced filaggrin (FLG) expression in DNCB-applied skin sections. Moreover, agerarin decreased phosphorylation of JAK1 and JAK2 kinases to enhance FLG expression, which was reduced by TNFα+IFNγ and IL4+IL13 treatment, in HaCaT keratinocytes. These results demonstrate the feasibility of agerarin as a possible therapeutic against conditions of skin inflammation, such as atopic dermatitis, by improving the upregulation of FLG expression.
Keywords: Atopic ermatitis, 6,7-Dimethoxy-2,2-dimethyl-2H-chromene, Filaggrin, Janus kinase, Keratinocyte
https://doi.org/10.38212/2224-6614.1178.
(https://www.jfda-online.com/journal/vol28/iss3/6/)