Nanovesicles derived from protocorm-like bodies of Dendrobium officinal enhance corneal epithelial repair and reduce inflammation in dry eye disease models

Bao Yuqing^a,b,d, Peng Chengyu^a,b,c,d, Zhang Fenglei^c,d,e, Zheng Yuqiang^c,d,e, Zhang Mingqi^c,d,e,**,1, He Wei^{a,b,c,d,e,*,1}

^a Jinzhou Medical University, China

^b He Eye Specialist Hospital, China

^c Stem Cell Center of Precision Medicine Innovation Institute, He University, Chinad Liaoning Key Lab of Ophthalmic Stem Cells, He University, China

^e School of Pharmacy, He University, China

Plant-derived nanovesicles (PDNVs) offer a promising, biocompatible therapeutic platform. In this study, we isolated nanovesicles from protocorm-like bodies of Dendrobium officinale (PLBs-NVs) and evaluated their efficacy in dry eyedisease (DED) models. PLBs-NVs protected human corneal epithelial cells against hyperosmotic stress, supported wound repair, and inhibited inflammation in vitro. In a desiccating stress-induced DED mouse model, topical PLBs-NVstreatment significantly restored tear secretion, reduced corneal epithelial damage, and lowered levels of proinflammatory cytokines. These results suggest that PLBs-NVs are a safe, natural, and effective therapeutic strategy for DED, sup-porting their potential for future clinical translation in ocular surface disorders.