Anti-neuroinflammatory polyketides from the medicinal herb-derived fungal strain Didymocyrtis brachylaenae Km1530

Chia-Hao Chang^a, Yun-Rong Zhang^a, Shu-Jung Huang^a, George Hsiao^b,c, Wei-Chiung Chi^d, Andrea Gu^a, Yu-Wei Lai^e, Tzong-Huei Lee^a,f,*

^a Institute of Fisheries Science, College of Life Science, National Taiwan University, Taipei 106319, Taiwan

^b Department of Pharmacology, School of Medicine, Taipei Medical University, Taipei 110301, Taiwan

^c Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110301, Taiwand Department of Food Science, National Quemoy University, Kinmen 892009, Taiwan

^e Center of General Education, National Taipei University, New Taipei 273303, Taiwan

^f Department of Life Science, College of Life Science, National Taiwan University, Taipei 106319, Taiwan

A series of chromatographic separation on the liquid-state fermented products of Didymocyrtis brachylaenae Km1530 derived from the littoral medicinal herb Atriplex maximowicziana Makino, resulted in the isolation of compounds 1—13. Their structures were determined by spectroscopic analysis as five previously unreported C13 polyketides, namely brachylactones A—D (1—4) and brachic acid (5), along with eight known compounds 6—13. Among these, brachy-lactones A (1) and B (2) exhibited nitric oxide production inhibitory activity in lipopolysaccharide-induced murine BV- 2 microglial cells at a concentration of 20 μM without any cytotoxicity.