Simultaneously qualitative and quantitative analysis of the representative components in Kadsura heteroclita stem by UHPLC-Q-Orbitrap HRMS

Qian-qian Liu ^a,1, Bin Li ^b,1, Kang Sun ^a, Cai-hong Li ^a, Nuzhat Shehla ^b, Yu-pei Yang ^b, Liang Cao ^b, Wei Wang ^b,**, Rong-xia Liu ^a,*

a School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University, Yantai 264005, China

b School of Pharmacy, TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Materia Medica Research Institute, Hunan University of Chinese Medicine, Changsha 410208, China d Department of Dermatology, Xiangya Hospital, Central South University, Changsha, 410008, China

Kadsura heteroclita (Roxb) Craib stem (KHS) is amedicinal plant used for the treatment of rheumatismarthritis diseases in Tujia ethnomedicine. Thus far, the complex chemical compositions in KHS are not clear, and the levels of the major compounds in KHS are not well understood. In this study, a novel UHPLC-Q-Orbitrap HRMS method was established for the simultaneous quali-quantitative analysis of KHS. A total of 204 compounds were identified, including triterpenoids, lignans, sesquiterpenes, fatty acids, phenolic acids, and flavonoids, more than 100 of which were first discovered in KHS. Using the same method, 12 representative bioactive components were successfully quantified. The method was fully validated by linearity, LOD, LOQ, precision, stability, recovery, and matrix effects, and it was applied to quantify the 12 representative compounds in 4 batches ofKHS.As thismethod enables retrospective data analysis and has no upper limit to the number of analytes in a single run, it can be applied to quantify more active components of KHS in the future.

Keywords: Identification, Kadsura heteroclita, Quantification, UHPLC-Q-Orbitrap HRMS

https://doi.org/10.38212/2224-6614.3348

 (https://www.jfda-online.com/journal/vol29/iss2/11)