Journal of Food and Drug Analysis (JFDA)
【Update Date:2005-06-07】unit:
Determination of Adenosine, Cordycepin and Ergosterol Contents in Cultivated Antrodia camphorata by HPLC MethodCHIEN-YU CHANG1,2, MING-YONG LUE3 AND TZU-MING PAN1*
1. Institute of Microbiology and Biochemistry, National Taiwan University, 1, Sec. 4, Roosevelt Rd., Taipei City 115, Taiwan, R.O.C.2. Taiwan Sugar Research Institute, Tainan, Taiwan, R.O.C.3. Department of Cosmetic Science, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan, R.O.C.(Received: March 3, 2005; Accepted: May 26, 2005)ABSTRACT
The concentrations of adenosine and cordycepin, 3'-deoxyadenosine in the hot water extract and ergosterol in the ethanol extract of a cultivated Antrodia camphorata were measured by high performance liquid chromatography (HPLC) method. The procedure were carried out on a reversed-phase C18 column by linear gradient elution, using four eluents as mobile phase and by isocratic elution, using 100% methanol as mobile phase, respectively. The analysis was completed within 20 min. The application of this method for quantitation of adenosine and cordycepin in 5-L and 100-L fermentation from two A. camphorata strains BCRC 35396 and BCRC 35398 were described. C omparison of adenosine, cordycepin and ergosterol contents were made with different reatment of 700-L fermentation broth.Key words: adenosine, cordycepin, ergosterol, Antrodia camphorata, high performance liquid chromatography (HPLC) method