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01.HPLC Assays of Naringin and Hesperidin in Chinese Herbs and Serum

【Update Date:1997-10-01】 unit:

HPLC Assays of Naringin and Hesperidin in Chinese Herbs and Serum

C.W. Chang1 , S.L. Hsiu2, P.P. Wu2, S.C. Kuo1 and P.D.L. Chao2

1.Graduate Institute of Pharmaceutical Chemistry, China Medical College, Taichung, Taiwan, R.O.C.
2. School of Pharmacy, China Medical College, Taichung, Taiwan, R.O.C.


ABSTRACT

   High performance liquid chromatographic methods for simultaneous determination of naringin and hesperidin in Chinese herbs and serum were established. By using a LiChrospher RP-18 column, the herbal extracts were separated by gradient elution with water-acetonitrile(0-10 min 80:20, 14-25 min 63:37) and the serum sample was separated by isocratic elution with water-acetonitrile(80:20) as the mobile phase. Both methods employed a flow rate of 1.0 ml/min with detection at 270 nm. Ethylparaben and vanillin were used as the internal standards in herbal extracts and in serum, respectively. The precision and accuracy of these methods were satisfactory for intraday and interday assays. The recoveries of naringin and hesperidin were almost quantitative from serum and from herbs including Fructus Aurantii Immaturus, Fructus Citri Immaturus Exsiccatus and Pericarpium Citri Chachiensis.

Key words: naringin, hesperidin, HPLC, herb, serum