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Articles & Issues

10. Analysis of Bakuchiol, Psoralen and Angelicin in Crude Drugs and Commercial Concentrated Products of Fructus Psoraleae

【Update Date:2007-12-13】 unit:
Analysis of Bakuchiol, Psoralen and Angelicin in Crude Drugs and Commercial Concentrated Products of Fructus Psoraleae

CHWAN-FWU LIN1,4, YU-LING HUANG2, MEI-YIN CHIEN3, SHUENN-JYI SHEU1
AND CHIEN-CHIH CHEN2*

1. Department of Chemistry, National Taiwan Normal University, Taipei, Taiwan (R.O.C.)
2. National Research Institute of Chinese Medicine, 155-1, Sec. 2, Li Nung St., Peitou, Taipei, Taiwan (R.O.C.)
3. Ko Da Pharmaceutical Co. Ltd., 20-1, Industrial 3 Rd., Ping-Cheng City, Tao-Yuan, Taiwan (R.O.C.)
4. Department of Cosmetic Science, Chang Gung Institute of Technology, Tao-Yuan, Taiwan (R.O.C.)

ABSTRACT

   The goal of this study was to develop a HPLC method for simultaneously determining bakuchiol, psoralen and angelicin in Fructus Psoraleae (Buguzhi, the fruits of Psoralea corylifolia) and its commercial concentrated products.  Extracted samples were analyzed by using a reverse-phase column (Cosmosil 5C18-AR-II) and eluting with a gradient mobile phase consisting of 20% acetonitrile to acetonitrile at a flow rate of 1.0 mL/min with detection at a wavelength of 254 nm.  The relative standard deviations of the marker substances on the basis of peak-area ratios in intraday and interday analyses were 0.3~1.5% and 0.2~1.0%, respectively.  Bakuchiol, psoralen and angelicin contents were 36.2~71.0, 2.5~13.0 and 2.2~9.2 mg/g for ten raw material samples of Fructus Psoraleae, and 0.6~21.1, 0.6~5.2 and 0.6~5.3 mg/g for eight commercial concentrated products of Fructus Psoraleae, respectively.

Key words: Psoralea corylifolia, HPLC, bakuchiol, psoralen, angelicin