Bighead Carp Meat Adulterated in Commercial Milkfish Ball Products
YU-JUN CHEN1, KEN-MEN HUANG2, HSIAO-CHIN JEN1, MING-WEI SU1, SHIU-MEI LIU2 AND DENG-FWU HWANG1*
1.Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan (R.O.C.)
2.Institute of Marine Biology, National Taiwan Ocean University, Keelung, Taiwan (R.O.C.)
(Received: August 15, 2008; Accepted: March 19, 2009)
ABSTRACT
Attempts were made to identify the species of raw materials of milkfish ball products. A 466-bp fragment could be amplified by polymerase chain reaction (PCR) using a pair of primer (L14735/H15149ad). Sequences of cytochrome b gene (cyt b) were determined in 3 fish species including milkfish (Chanos chanos), bighead carp (Hypophthalmichthys nobilis) and grass carp (Ctenopharyngodon idella). These three fish species contained distinguishable sequences. In addition, RFLP analysis of the PCR products with endonuclease HpaII showed unique restriction maps for these 3 fish species. Based on gene sequencing and PCRRFLP analyses, we have identified one commercial milkfish ball be intentionally adulterated with bighead carp.
Key words: cytochrome b gene, milkfish, bighead carp, grass carp, PCR-RFLP