Determination of Flavonoids in Daphnis Genkwae Flos by High Performance Liquid Chromatography
JER-HUEI LIN, YA-TZE LIN, YI-NING CHIOU, KUO-CHING WEN* AND CHUN-HENG LIAO
National Laboratories of Foods and Drugs, Department of Health, Executive Yuan 161-2, Kuen-Yang Street, Nankang, 11513, Taipei, Taiwan
(Received: August 21, 2000; Accepted: November 22, 2000)
ABSTRACT
In order to evaluate the quality of Daphnis Genkwae Flos, a high performance liquid chromatographic method (HPLC) was developed. Six flavonoid constituents from Daphnis Genkwae Flos, genkwanin 5-O-β-D-primeveroside (1), genkwanin 5-O-β-D-glucoside (2), genkwanin (3), potassium apigenin 7-O-β-D-glucuronate (4), apigenin (5) and tiliroside (6) determined by this simple, rapid and accurate method.
Samples were analyzed using a Cosmosil 5C18-AR reversed phase column by gradient elution with varied proportion of 1.0 % (v/v) acetic acid and acetonitrile as mobile phase at 254 nm. Sulfamethoxypyridazine was used as the internal standard.
Regression equations revealed the linear relationship (correlation coefficients: 0.9994~0.9999) between the peak-area ratios flavonoid to internal standard and concentrations of flavonoids. The recoveries of six flavonoids were between 100.9~106.1%. The relative standard deviations of six flavonoids ranged between 0.49~3.46% (intraday) and 0.52~4.61% (interday). The contents of the six flavonoids in sixteen crude drugs of Daphnis Genkwae Flos were 1 0.13~0.40%, 2 0.02~0.12%, 3 0.24~0.56%, 4 0.16~1.19%, 5 0.30~0.73% and 6 0.15~0.57%, respectively.
Key words: Daphnis Genkwae Flos, flavonoids,HPLC