Preparative Isolation and Gas Chromatography-Mass Spectrometry Analysis of Triterpenoids in Kansui Radix
JER-HUEI LIN*, YOE-RAY KU, YA-TZE LIN, SHU-FANG TENG, KUO-CHING WEN AND CHUN-HENG LIAO
National Laboratories of Foods and Drugs, Department of Health, Executive Yuan, 161-2, Kuen-Yang Street, Nankang, Taipei 115, Taiwan, Republic of China
(Received: July 10, 2000; Accepted: October 3, 2000)
ABSTRACT
Two triterpenoids, euphol and tirucallol were isolated from Kansui Radix (Euphorbia kansui Liou, Euphorbiaceae) by a modified isolation method. These constituents were used as marker substances for quality control of Kansui Radix using a home-developed gas chromatography-mass spectrometry (GC-MS) method.
The linear calibration ranges were 50.0-400.0 mg/mL (r=0.9988) for euphol and 20.0-200.0 mg/mL (r=0.9976) for tirucallol. The relative standard deviations of two marker substances for intraday and interday analyses were 0.90-3.07% and 3.93-8.17%, respectively. Recoveries were euphol 98.3±1.2%, and tirucallol 84.8±1.0%. The contents of two marker substances in seven crude drugs of Kansui Radix were euphol 0.10-0.19%, and tirucallol 0.05-0.07%.
Key words: pharmaceutical analysis, Kansui Radix, tirucallol, euphol, GC-MS