Determination of Naringin in Marketed Fruits, Juices and Candy from Shaddock
SU-LAN HSIU*, TANG-YEN HWANG2, YU-CHI HOU3, CHIH-WAN TSAO2 AND PEI-DAWN LEE CHAO1
1.School of Pharmacy
2.Graduate Institue of Pharmaceutical Chemistry
3.Graduate Institute of Chinese Parmaceutical Sciences
China Medical College, Taichung, Taiwan, R.O.C.
ABSTRACT
High performance liquid chromatography was used to determine the contents of naringin in the exocarp, mesocarp and pulp of shaddock and white shaddock sold in markets. The analysis was carried out on an Hypersil ODS column (5μm, 250 x 4.6mm), using water-acetonitrile (81:19) as mobile phase at a flow rate of 0.8ml/min. The detection was set a 280nm and 6,7-dimethoxycoumarin was used as the internal standard.
The precision of this method (C.V. 0.3 ~ 5.0%) and the recoveries (76.9 –102.2%) were satisfactory for various shaddock fruits and juices. The results indicated the contents of naringin in mesocarp were the highest for both shaddock and white shaddock (23.4mg/g and 16.8mg/g, respectively), whereas exocarp and pulp contained a lesser amount. Only a small amount of naringin was found in shaddock juices and shaddock candy.
Key words: naringin, shaddock, high performance liquid chromatography (HPLC)