A New Method for Determining Heme Iron in Pork Using Solid Phase Extraction and Graphite Furnace Atomic Absorption Spectrophotometer
Syr-Song Chen1*, Wei-Hsien Chang2 and Shin-Shou Chou1
1. National Laboratories of Foods and Drugs, Department of Health, Executive Yuan
Graduate Institute of Food Science and Technology, National Taiwan University
ABSTRACT
A new method for selective determination of heme iron in pork was developed. First heme iron in pork was extracted with 80% acetone acidified with 2% hydrochloric acid to below pH 1.0. After adjusting the concentration of acetone to 40%, the solution was passed through a Sep-Pak C18 cartridge. Heme was adsorbed on the cartridge, and was then eluted with 80% acetone. The eluent was analyzed for heme iron with a graphite furnace atomic absorption spectrophotometer. Recovery studies were performed by adding 1.75, 2.50 and 3.75 μg HFe/g myoglobin and 3.00, 7.00 and 10.00 μg HFe/g hemoglobin into the pork. Recoveries were in the range of 97.9～99.3%.
Key words: solid phase extraction, graphite furnace atomic absorption spectrophotometer (GFAAS), pork, heme iron (HFe)