Development of a High Performance Liquid Chromatographic Method for Detection of Sulfonamide Residues in Egg

SHU-CHI TSAI*, YUNG-HWA RAU, SU-CHIEN HSU, CHI-CHANG CHOU AND WEIGUANG FU

Food Industry Research & Development Institute, Hsinchu, Taiwan, R.O.C.

ABSTRACT

   A rapid and precise method was developed for detection of three sulfonamide compounds (SMT, SMM and SDM) by using high performance liquid chromatography (HPLC). Egg samples were extracted with acetone and dichloromethane solvent. The extract was then cleaned up and concentrated by a silica cartridge and a C18 cartridge with low flow rates before being subjected to HPLC analysis. Recoveries for spiked egg samples were above 90% for all three specific sulfonamide compounds. When the method was applied to 150 commercial egg samples, only two samples were detected with sulfonamide residue concentrations over 0.1 ppm.

Key words : sulfonamide, sulfadrug, sulfamethazine, sulfamonomethoxin, sulfadimethoxine, egg, HPLC, silica cartridge, C18 cartridge