Studies on The Promotion of Transformation of Initiated Rat Liver Epithelial Cell by Phenobarbital, Butylated Hydroxyanisole and Butylated Hydroxytoluene

HSHINN-HSHIUNG TSENG AND *WENG-FOUNG HUANG

National Laboratories of Foods and Drugs, Department of Health, Executive Yuan, 161-2, Kuen Yang Street, Nan Kang, Taipei, Taiwan, R.O.C.
*Graduate Institute of Health & Welfare Policy, National Yang-Ming University, Shih-Pai, Taipei 11221, Taiwan, R.O.C.

ABSTRACT

   Newborne wistar rats were injected (i.p) with a single dose of 2-acetylaminofluorene (2-AAF) for in vivo model of initiation, one week later, the liver epithelial cells were obtained by a trypsinization of the pooled livers and used for in vitro model to evaluate the promotion of cell transformation by phenobarbital (PB), butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). In the absence of promoting treatment, the initiated liver epithelial cells remained untransformation at least during 20 weeks of culture. At the certain concentration, not only the growth and proliferative activity of the initiated liver epithelial cells were effected but also the marker of the transformation and malignant tumor such as the activity of r-glutamyltranspeptidase, epidermal growth factor binding and DNA increasing were induced in a dose-response manner by in vivo-in vitro combination model.

Key words : phenobarbital(PB), butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), cell transformation