Quantitative Analysis of Paeoniflorin, Geniposide and Glycyrrhizin in Jing-Jieh-Lian-Chyau-Tong by High Performance Liquid Chromatography

SHION-JANE LIN, CHENG-YU HUANG, KUO-CHING WEN AND ERICK TSI-TEE SUEN

National Laboratories of Foods and Drugs, Department of Health, Executive Yuan

ABSTRACT

   High Performance Liquid Chromatographic methods for determination of paeoniflorin in Pasoniae Radix, geniposide in Gardenia Radix and glycyrrhizin in Glycyrrhizae Radix were developed for the quality control of traditional Chinese medicinal prescriptions Jing-Jieh-Lian-Chyau-Tong. Samples were extracted with 70% methanol by ultrasonic shaking for 30 minutes. The extract was separated by Inertsil 5 ODS-2 column with water-acetonitrile (85:15) as the mobile phase at a flow-rate of 1.0 ml/min. for paeoniflorin and geniposide, while for glycyrrhizin, it was separated by Lichrospher 100 RP-18 column with 1% acetic acid-acetonitrile (65:35) as the mobile phase at a flow-rate of 1.0 ml/min. The detecting wavelength was 254 nm with methylparaben and butylparaben used as the internal standards respectively. The calibration curves were linear in the range of 0.025-0.200 mg/ml for paeoniflorin; 0.02-0.10 mg/ml for geniposide and 0.016-0.150 mg/ml for glycyrrhizin. The recovery for each constituent from this prescription was 100.0% for paeoniflorin; 99.6% for geniposide and 99.9% for glycyrrhizin and the coefficient of variation was lower than 1.0%. The contents of these preparation constituents were not affected by treatment with concentration under reduced pressure, spray-drying or lyophilization respectively. The difference between the highest and lowest content of paeoniflorin, geniposide and glycyrrhizin were 4.2, 4.8 and 4.6-fold respectively.

Key Words : Paeoniflorin, Geniposide, Glycyrrhizin, HPLC.