Molecular Recognition and Identification of Sulfapyridine and Sulfadimethoxine by High Performance Liquid Chromatography with Molecularly Imprinted Polymer as the Stationary Phase
CHIN-YIN HUNG1, YUN-TZU HUANG2 AND CHING-CHIANG HWANG3*
1. Department of Biotechnology, National Formosa University, Yunlin, Taiwan (R.O.C.)
2. Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan (R.O.C.)
3. Department of Life Science, Mingdao University, ChangHua, Taiwan (R.O.C.)
(Received: June 26, 2007; Accepted: December 18, 2007)
ABSTRACT
Molecular imprinting technique is becoming an important tool for determining sulfonamides because of its simplicity and high separation efficiency. The creation of molecularly imprinted polymers (MIPs) for recognition of sulfapyridine and sulfadimethoxine is reported. Polymers were prepared using methacrylic acid (MAA) as the functional monomer and sulfapyridine as the template during imprinting. The recognition properties of the polymers were assessed by high performance liquid chromatography (HPLC) for their ability to retain the templates and analogous analytes. Retention time of sulfapyridine and sulfadimethoxine was approximately 8.24 and 3.89 min, respectively. In order to compare the chromatographic data from the stationary phase, both retention factor (k’) and separation factor (α) were given. The value of 2.39 for separation factor indicated that the MIP was able to recognize structurally subtle differences from the template molecule. Our results are discussed with regard to the amount of template, the compositions of the chromatographic mobile phase and the adsorption capacity.
Key words: molecularly imprinted polymers (MIPs), recognition, sulfapyridine, sulfadimethoxine, high performance liquid chromatography (HPLC)